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首頁(yè) 全所PI名錄
  • 惠靜毅
  • 研究員，研究組長(zhǎng)，博士生導(dǎo)師
  • E-mail: jyhui@@sibcb.ac.cn
  • 實(shí)驗(yàn)室主頁(yè): 
    個(gè)人簡(jiǎn)介：

  • 　　1993年復(fù)旦大學(xué)微生物系畢業(yè)，獲學(xué)士學(xué)位。1999年德國(guó)Free University Berlin畢業(yè)，獲碩士學(xué)位。2003年德國(guó)Justus-Liebig-University Giessen畢業(yè)，獲博士學(xué)位。2008年回國(guó)后，任中科院上海生科院生化與細(xì)胞所研究員，研究組長(zhǎng)。

    社會(huì)任職：
  •  
    研究方向：
  • RNA加工的調(diào)控與功能
    研究工作：

  • 　　RNA結(jié)合蛋白是一類能夠結(jié)合單鏈或雙鏈RNA的蛋白質(zhì)。越來(lái)越多的實(shí)驗(yàn)結(jié)果表明RNA結(jié)合蛋白參與了RNA代謝過(guò)程中幾乎各個(gè)步驟，在各種生命活動(dòng)中發(fā)揮重要功能。RNA結(jié)合蛋白編碼基因的突變、擴(kuò)增、缺失或者RNA結(jié)合蛋白的表達(dá)與性質(zhì)變化與許多人類疾病密切相關(guān)。我們通過(guò)采用多學(xué)科手段，探索RNA結(jié)合蛋白調(diào)控基因表達(dá)的分子機(jī)制以及在人類疾病中這些機(jī)制是如何失調(diào)的。我們特別關(guān)注RNA結(jié)合蛋白調(diào)控RNA加工的機(jī)制與功能。RNA加工是RNA成熟并發(fā)揮功能所必不可少的步驟，也是基因表達(dá)過(guò)程中的一個(gè)重要調(diào)控層面，在這一方向上的研究?jī)?nèi)容主要包括：1. RNA結(jié)合蛋白調(diào)控mRNA可變剪接的分子機(jī)理研究;2. RNA結(jié)合蛋白在腫瘤發(fā)生發(fā)展中的機(jī)制與功能研究;3. 神經(jīng)發(fā)育紊亂中RNA與染色質(zhì)相互作用的調(diào)控機(jī)制研究等。

    承擔(dān)科研項(xiàng)目情況：
    代表論著：
    1. Wang JZ*, Zhu H*, You P*, Liu H*, Wang WK*, Fan X, Yang Y, Xu K, Zhu Y, Li Q, Wu P, Peng C, Wong CC, Li K, Shi Y, Zhang N, Wang X, Zeng R, Huang Y, Yang L#, Wang Z#, Hui J#. Upregulated YB-1 protein promotes glioblastoma growth through a YB-1/CCT4/mLST8/mTOR pathway. ( 2022) J Clin Invest, Apr 15;132(8):e146536
    2. Jiang Y*, Fu X*, Zhang Y*, Wang SF, Zhu H, Wang WK, Zhang L, Wu P, Wong CCL, Li J, Ma J, Guan JS, Huang Y#, Hui J#. Rett syndrome linked to defects in forming the MeCP2/Rbfox/LASR complex in mouse models. (2021) Nat Commun, 12, 5767
    3. Pan X, Zhao J, Zhou Z, Chen J, Yang Z, Wu Y, Bai M, Jiao Y, Yang Y, Hu X, Cheng T, Lu Q, Wang B, Li CL, Lu YJ, Diao L, Zhong YQ, Pan J, Zhu J, Xiao HS, Qiu ZL, Li J, Wang Z, Hui J, Bao L, Zhang X. 5'-UTR SNP of FGF13 causes translational defect and intellectual disability. (2021) Elife, 10: e63021
    4. Park E, Jiang Y, Hao L, Hui J, Xing Y. Genetic variation and microRNA targeting of A-to-I RNA editing fine tune human tissue transcriptomes. (2021) Genome Biol, 22, 77
    5. Wang JZ*, Fu X*, Fang Z*, Liu H, Zong FY, Zhu H, Yu YF, Zhang XY, Wang SF, Huang Y, Hui J#. QKI-5 regulates the alternative splicing of cytoskeletal gene ADD3 in lung cancer. (2021) J Mol Cell Biol, 13, 347-360
    6. Wang X, Li ZT, Yan Y, Lin P, Tang W, Hasler D, Meduri R, Li Y, Hua MM, Qi HT, Lin DH, Shi HJ, Hui J, Li J, Li D, Yang JH, Lin J, Meister G, Fischer U, Liu MF. LARP7-Mediated U6 snRNA Modification Ensures Splicing Fidelity and Spermatogenesis in Mice. (2020) Mol Cell, 77, 999-1013
    7. Yang XJ, Zhu H, Mu SR, Wei WJ, Yuan X, Wang M, Liu Y, Hui J#, Huang Y#. Crystal structure of a Y-box binding protein 1 (YB-1)-RNA complex reveals key features and residues interacting with RNA. (2019) J Biol Chem, 294, 10998-11010
    8. Müller-McNicoll M, Rossbach O, Hui J, Medenbach J. Auto-regulatory feedback by RNA-binding proteins. (2019) J Mol Cell Biol, 11, 930-939
    9. Wu DR*, Gu KL*, Yu JC, Fu X, Wang XW, Guo WT, Liao LQ, Zhu H, Zhang XS, Hui J#, Wang Y#. Opposing roles of miR-294 and MBNL1/2 in shaping the gene regulatory network of embryonic stem cells. (2018) EMBO Rep 19, pii: e45657
    10. Wang F, Fu X, Chen P, Wu P, Fan XJ, Li N, Zhu H, Jia TT, Ji H, Wang ZF, Wong CCL, Hu RG#, Hui J#. SPSB1-mediated HnRNP A1 ubiquitylation regulates alternative splicing and cell migration in EGF signaling. (2017) Cell Res, 27, 540-558 (highlighted by Fu XD. Both sides of the same coin: Rac1 splicing regulating by EGF signaling. (2017) Cell Res, 27, 455-456)
    11. Yuan H, Li N, Fu D, Ren J, Hui J, Peng J, Liu Y, Qiu T, Jiang M, Pan Q, Han Y, Wang X, Li Q, Qin J. Histone methyltransferase SETD2 modulates alternative splicing to inhibit intestinal tumorigenesis. (2017) J Clin Invest, 127, 3375-3391
    12. Wang F, Song W, Zhao HM, Ma YN, Li YX, Zhai D, Pi JN, Si YM, Xu JY, Dong L, Su R, Zhang MM, Zhu Y, Ren XX, Miao F, Liu WJ, Li FJ, Zhang JW, He AB, Shan G, Hui J, Wang LF, Yu J. The RNA-binding protein QKI5 regulates primary miR-124-1 processing via a distal RNA motif during erythropoiesis. (2017) Cell Res, 27, 416-439
    13. Li F, Yi P, Pi J, Li L, Hui J, Wang F, Liang A, Yu J. QKI5-mediated alternative splicing of the histone variant macroH2A1 regulates gastric carcinogenesis. (2016) Oncotarget, 7, 32821-32834
    14. Wu SL, Fu X*, Huang J*, Jia TT, Zong FY, Mu SR, Zhu H, Yan Y, Qiu S, Wu Q, Yan W, Peng Y, Chen J, Hui J#. Genome-wide analysis of YB-1-RNA interactions reveals a novel role of YB-1 in miRNA processing in glioblastoma multiforme. (2015) Nucleic Acids Res, 43, 8516-8528
    15. Guo R, Zheng L, Park JW, Lv R, Chen H, Jiao F, Xu W, Mu S, Wen H, Qiu J, Wang Z, Yang P, Wu F, Hui J, Fu X, Shi X, Shi YG, Xing Y, Lan F, Shi Y. BS69/ZMYND11 reads and connects histone H3.3 lysine 36 trimethylation-decorated chromatin to regulated pre-mRNA processing. (2014) Mol Cell, 56, 298-310
    16. Zong FY, Fu X, Wei WJ, Luo YG, Heiner M, Cao LJ, Fang Z, Fang R, Lu D, Ji H, Hui J#. The RNA-binding protein QKI suppresses cancer-associated aberrant splicing. (2014) PLOS Genet, 10, e1004289
    17. Jiang Y, Hui J#. Chromatin structure and mRNA alternative splicing. (2014) Chem Life, 4, 449-455 (invited review)
    18. Wei WJ*, Mu SR*, Heiner M, Fu X, Cao LJ, Gong XF, Bindereif A, Hui J#. YB-1 binds to CAUC motifs and stimulates exon inclusion by enhancing the recruitment of U2AF to weak polypyrimidine tracts. (2012) Nucleic Acids Res, 40, 8622-8636
    19. Huang Y, Li W, Yao X, Lin QJ, Yin JW, Liang Y, Heiner M, Tian B, Hui J, Wang G. Mediator complex regulates alternative mRNA processing via the MED23 subunit. (2012) Mol Cell, 45, 459-469
    20. Heiner M, Hui J, Schreiner S, Hung LH, Bindereif A. HnRNP L-mediated regulation of mammalian alternative splicing by interference with splice site recognition. (2010) RNA Biol, 7, 56-64
    21. Kishore S, Khanna A, Zhang Z, Hui J, Balwierz PJ, Stefan M, Beach C, Nicholls RD, Zavolan M, Stamm S. The snoRNA MBII-52 (SNORD 115) is processed into smaller RNAs and regulates alternative splicing. (2010) Hum Mol Genet, 19, 1153-1164
    22. Ro？bach O, Hung LH, Schreiner S, Grishina I, Heiner M, Hui J, Bindereif A. Auto- and crossregulation of the hnRNP L proteins by alternative splicing. (2009) Mol Cell Biol, 29, 1442-1451
    23. Hui J#. Regulation of mammalian pre-mRNA splicing. (2009) Sci China C Life Sci, 52, 253-260 (invited review)
    24. Hui J, Kishore S, Khanna A, Stamm S. Analysis of alternative splicing with microarrays. (2009) In Bioinformatics for Systems Biology (Krawetz SA ed.), Humana Press, pp267-279
    25. Hung LH*, Heiner M*, Hui J*, Schreiner S, Benes V, Bindereif A. Diverse roles of hnRNP L in mammalian mRNA processing: a combined microarray and RNAi analysis. (2008) RNA, 14, 284-296
    26. Lorenz M, Hewing B, Hui J, Zepp A, Baumann G, Bindereif A, Stangl V, Stangl K. Alternative splicing in intron 13 of the human eNOS gene: a potential mechanism for regulating eNOS activity. (2007) FASEB J, 21, 1556-1564
    27. Hui J, Bindereif A. Alternative pre-mRNA splicing in the human system: unexpected role of repetitive sequences as regulatory elements. (2005) Biol Chem, 386, 1265-1271 (invited review)
    28. Hui J*, Hung LH*, Heiner M, Schreiner S, Neumuller N, Reither G, Haas SA, Bindereif A. Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. (2005) EMBO J, 24, 1988-1998
    29. Palfi Z, Hui J, Bindereif A. Biotin-based affinity purification of RNA-protein complexes. (2004) In Handbook of RNA Biochemistry, (Westhof E, Bindereif A, Sch？n A, Hartmann RK eds), Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, pp676-693
    30. Hui J, Reither G, Bindereif A. Novel function role of CA repeats and hnRNP L in RNA stability. (2003) RNA, 9, 931-936
    31. Hui J, Stangl K, Lane WL, Bindereif A. HnRNP L stimulates splicing of the eNOS gene by binding to variable-length CA repeats. (2003) Nat Struct Biol, 10, 33-37. (Comment by Bilbao D, Valcárcel J. Getting to the heart of a splicing enhancer. (2003) Nat Struct Biol, 10, 6-7)
    32. Yang J, Hui J, Li G, Wang Y, Yuan H, Li Y. Expression of the recombinant hepatitis B virus surface antigen carrying preS epitopes in Pichia pastoris. (2000) Acta Biochimica et Biophysica Sinica, 32, 39-144
    33. Hui J, Li G, Kong Y, Wang Y. Expression and characterization of chimeric hepatitis B surface antigen particles carrying preS epitopes. (1999) J Biotechnol, 72, 49-59
    34. Hui J, Mancini M, Li G, Wang Y, Tiollais P, Michel ML. (1999) Immunization with a plasmid encoding a modified hepatitis B surface antigen carrying the receptor binding site for hepatocytes. Vaccine, 17, 1711-1718
    35. Hui J, Li G, Kong Y, Wang Y. DNA-mediated immunization against hepatitis B surface antigen carrying preS epitopes. (1999) Chinese Science Bulletin, 44, 620-62
    36. Hui J, Li G, Kong Y, Wang Y. A modified HBsAg containing the preS1 and preS2 epitopes. (1998) Sci China C Life Sci, 41, 56-63
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    研究組成員：